Smoking and Brain Neoplasm: An Immunohistochemical Data Evaluating Caspase-3 and MMP-2 in Rat Brain

Background and Aim: Smoking has been extensively investigated in oncology, controversial associations with brain tumor incidence have reported. Caspase-3 matrix metalloproteinases (MMPs) belong to an important cascade vasculogenesis. We aim study the smoking impact on these signaling molecules nontumoral rat tissue. Methods Materials/Patients: A total of 60 Wistar rats were divided into two groups: treatment (cigarette smoke/electronic cigarette) control groups subgroups male female rats. After general anesthesia decapitation, their brains collected, 3-μm thick coronal sections prepared. Following immunohistochemical staining rabbit anti-MMP-2 anti-caspase-3 antigens, protein expressions analyzed by selecting fields at 400x magnification. Results: Our data suggest that expression MMP-2 was not significantly different between studied groups. However, significant inductive activity cigarette observed caspase-3. Conclusion: Cigarette indicates pro-tumoral normal tissue activation caspase-3